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receptortransporting protein 1 (rtp1s)  (Matsunami Glass)

 
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    Matsunami Glass receptortransporting protein 1 (rtp1s)
    Receptortransporting Protein 1 (Rtp1s), supplied by Matsunami Glass, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
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    Endpoint measurement of the binding affinity of odor molecules to OR expressed on heterologous cells. To promote OR expression on the surface of heterologous cells, a signal peptide (Rho-tag) is added to the N -terminus and the OR chaperones RTPs (RTP1, RTP1S, RTP2) and REEP1 and Gα protein chaperone Ric-8B are coexpressed. Binding of the odor molecule causes OR to activate adenylate cyclase (AC) via trimeric G proteins to produce cAMP. The amount of cAMP is assayed by endpoint measurement of chemiluminescence using GloSensor TM and is determined as the binding affinity between the odor molecules and OR.

    Journal: Sensors (Basel, Switzerland)

    Article Title: Human Olfactory Receptor Sensor for Odor Reconstitution

    doi: 10.3390/s23136164

    Figure Lengend Snippet: Endpoint measurement of the binding affinity of odor molecules to OR expressed on heterologous cells. To promote OR expression on the surface of heterologous cells, a signal peptide (Rho-tag) is added to the N -terminus and the OR chaperones RTPs (RTP1, RTP1S, RTP2) and REEP1 and Gα protein chaperone Ric-8B are coexpressed. Binding of the odor molecule causes OR to activate adenylate cyclase (AC) via trimeric G proteins to produce cAMP. The amount of cAMP is assayed by endpoint measurement of chemiluminescence using GloSensor TM and is determined as the binding affinity between the odor molecules and OR.

    Article Snippet: Matsunami et al. found that human embryonic kidney-derived HEK293 cells introduced with the chaperones RTP1 (receptor-transporting protein 1), RTP2, and REEP1 (receptor expression-enhancing protein 1), also called Hana3A cells, greatly improved cell surface expression of ORs with an N -terminal Rho-tag (rhodopsin-derived signal peptide) [ ] ( ).

    Techniques: Binding Assay, Expressing